Original Article
 
Detection of basement membrane alterations in head and neck tissue biopsy using double immunostaining technique
Churaiorn Unhasuta1, Koson Intarasut1
1Institute of Pathology
Ministry of Public Health, Bangkok, Thailand

Article ID: 100020P16CU2018
doi: 10.5348/100020P16CU2018OA

Corresponding Author:
Churaiorn Unhasuta, MD
Institute of Pathology
2/2 Phayathai Road, Bangkok 10400, Thailand

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How to cite this article
Unhasuta C, Intarasut K. Detection of basement membrane alterations in head and neck tissue biopsy using double immunostaining technique. Edorium J Public Health 2018;5:100020P16CU2018.


ABSTRACT

Certain cases pose diagnostic difficulty when it comes to discriminating between early squamous cell carcinoma, reactive processes, and dysplasia located at the head and neck. The gold standard test is confirming invasion of tissues beyond basement membrane. This study sought to verify whether basement membrane markers and usage of cytokeratin double immunostaining can improve the process. In total, forty retrospective cases of both neck and head biopsies have been used as the basis for this study. Out of the total, there were fifteen invasive squamous cell carcinoma cases (SCC), fifteen in-situ carcinoma cases (CIS), and ten pseudoepitheliomatous hyperplasia cases (PEH). The entire case distribution has received evaluation via immunohistochemistry. For this purpose, Cytokeratin (CK), Laminin and Collagen IV monoclonal antibodies have been used. In all cases, it has been found that the surroundings of the invasive tumor, and especially those of the malignant cells at the front, showed discontinuities or total absence of laminin and collagen IV staining. Therefore, it has been concluded that laminin and collagen IV may be used in the future as secondary differential diagnostic criteria besides double immunostaining for histological differentiation between SCC, CIS, and PEH.

Keywords: Basement membrane, Carcinoma in situ, Collagen IV, Laminin, Pseudoepitheliomatous hyperplasia, Squamous cell carcinoma


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Acknowledgements
Thanks especially to Dr. Songkun Vinyoovattana, MD. and my colleagues who provided assistance, support, and encouragement in doing this research.
Author Contributions
Churaiorn Unhasuta – Substantial contributions to conception and design, Acquisition of data, analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Koson Intarasut – Substantial contributions to conception and design, Acquisition of data, Drafting the article, Final approval of the version to be published
Guarantor of Submission
The corresponding author is the guarantor of submission.
Source of Support
None
Consent Statement
Written informed consent was obtained from the patient for publication of this study.
Conflict of Interest
Author declares no conflict of interest.
Copyright
© 2018 Churaiorn Unhasuta et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.